Protocols

Nascent RNA labeling and purification 

The procedure labels and separates the nascent RNA fraction from steady state RNAs, using 4-thio-Uridine (4sU) pulse/chase experiments. Initially developed by Caitlin Roake.

08/19/2021 

Genotyping PCR of the human TERT promoter

The human TERT promoter is highly GC-rich, and unfriendly to routine PCR reactions. Here, we describe a procedure that was used to genotype this region, related to TERT Promoter Mutations (TPMs) and the TERT ATG. 

08/21/2021