Nascent RNA labeling and purification
The procedure labels and separates the nascent RNA fraction from steady state RNAs, using 4-thio-Uridine (4sU) pulse/chase experiments. Initially developed by Caitlin Roake.
Genotyping PCR of the human TERT promoter
The human TERT promoter is highly GC-rich, and unfriendly to routine PCR reactions. Here, we describe a procedure that was used to genotype this region, related to TERT Promoter Mutations (TPMs) and the TERT ATG.