Lu Chen Lab @ Fox Chase Cancer Center
solving disease one base pair at a time
Publications @
Fox Chase Cancer Center
2. Chen L ✉, Roake CM, Maccallini P, Bavasso F, Dehghannasiri R, Santonicola P, Mendoza-Ferreira N, Scatolini L, Rizzuti L, Esposito A, Gallotta I, Francia S, Cacchione S, Galati A, Palumbo V, Kobin MA, Tartaglia GG, Colantoni A, Proietti G, Wu Y, Hammerschmidt M, De Pittà C, Sales G, Salzman J, Pellizzoni L, Wirth B, Schiavi ED, Gatti M, Artandi SE, Raffa GD. TGS1 impacts snRNA 3'-end processing, ameliorates survival motor neuron-dependent neurological phenotypes in vivo and prevents neurodegeneration. Nucleic Acids Research 2022 Aug 10:gkac659. doi: 10.1093/nar/gkac659. Epub ahead of print. PMID: 35947650. (✉ co-corresponding)
1. Galati A, Scatolini L, Micheli E, Bavasso F, Cicconi A, Maccallini P, Chen L, Roake CM, Schoeftner S, Artandi SE, Gatti M, Cacchione S, Raffa GD. The S-adenosylmethionine analog sinefungin inhibits the trimethylguanosine synthase TGS1 to promote telomerase activity and telomere lengthening. FEBS Lett. 2022 Jan;596(1):42-52. doi: 10.1002/1873-3468.14240. Epub 2021 Dec 5. PMID: 34817067.
Publications
@Stanford University
STAR Protocols 2021; 2 (2), 100477
Analysis of RNA conformation in endogenously assembled RNPs by icSHAPE
Lu Chen ✉, Howard Y Chang, Steven E Artandi ✉
(✉ co-corresponding)
DNA Repair (Amst). 2021 Jan;97:103022.
A novel DDB2 mutation causes defective recognition of UV-induced DNA damages and prevalent equine squamous cell carcinoma
Lu Chen ✉, Rebecca R Bellone, Yan Wang, Moriel Singer-Berk, Kaoru Sugasawa, James M Ford, Steven E Artandi
(✉ corresponding)
Molecular Therapy. 2021 Mar 29;S1525-0016(21)00152-0.
Targeted replacement of full-length CFTR in human airway stem cells by CRISPR/Cas9 for pan-mutation correction in the endogenous locus
Sriram Vaidyanathan, Ron Baik, Lu Chen, Dawn T Bravo, Carlos J Suarez, Shayda M Abazari, Ameen A Salahudeen, Amanda M Dudek, Christopher A Teran, Timothy H Davis, Ciaran M Lee, Gang Bao, Scott H Randell, Steven E Artandi, Jeffrey J Wine, Calvin J Kuo, Tushar J Desai, Jayakar V Nayak, Zachary M Sellers, Matthew H Porteus
bioRxiv 2021.04.28.441728
Clonal inactivation of telomerase promotes accelerated stem cell differentiation
Kazuteru Hasegawa, Yang Zhao, Alina Garbuzov, M.Ryan Corces, Lu Chen, Peggie Cheung, Yuning Wei, Howard Y. Chang, Steven E. Artandi
bioRxiv 356782 (doi.org/10.1101/2020.10.27.356782)
TGS1 controls snRNA 3’ end processing, prevents neurodegeneration and ameliorates SMN-dependent neurological phenotypes in vivo
Lu Chen, CM Roake, P Maccallini, Bavasso F, R Dehghannasiri, Santonicola P, Mendoza-Ferreira N, Scatolini L, Rizzui L, Esposito A, Gallotta I, Francia S, Cacchione S, Hammerschmidt M, De Pittà C, Sales G, Salzman J, Pellizzoni L, Wirth B, Di Schiavi E, Gatti M, Artandi SE, Raffa GD
Cell Reports. 2020 Feb 4;30(5):1358-1372.e5.
Loss of human TGS1 hypermethylase promotes increased telomerase RNA and telomere elongation
Lu Chen, Caitlin M Roake, Alessandra Galati, Francesca Bavasso, Emanuela Micheli, Isabella Saggio, Stefan Schoeftner, Stefano Cacchione, Maurizio Gatti, Steven E Artandi, Grazia D Raffa
Molecular Cell. 2019 May 16;74(4):688-700.e3.
Disruption of Telomerase RNA Maturation Kinetics Precipitates Disease
Caitlin M Roake, Lu Chen, Ananya L Chakravarthy, James E Ferrell Jr, Grazia D Raffa, Steven E Artandi
Cell. 2018 Jun 28;174(1):218-230.e13.
An Activity Switch in Human Telomerase Based on RNA Conformation and Shaped by TCAB1
Lu Chen, Caitlin M Roake, Adam Freund, Pedro J Batista, Siqi Tian, Yi A Yin, Chandresh R Gajera, Shengda Lin, Byron Lee, Matthew F Pech, Andrew S Venteicher, Rhiju Das, Howard Y Chang, Steven E Artandi
Nature. 2018 Apr;556(7700):244-248.
Distributed hepatocytes expressing telomerase repopulate the liver in homeostasis and injury
Shengda Lin, Elisabete M Nascimento, Chandresh R Gajera, Lu Chen, Patrick Neuhöfer, Alina Garbuzov, Sui Wang, Steven E Artandi
Publications
@Stowers Institute for Medical Research /
Kansas University Medical Center
Proceedings of the National Academy of Sciences 2014; 111 (52), 18566-18571
Regulation of the Rhp26ERCC6/CSB chromatin remodeler by a novel conserved leucine latch motif
Lanfeng Wang, Oliver Limbo, Jia Fei, Lu Chen, Bong Kim, Jie Luo, Jenny Chong, Ronald C Conaway, Joan W Conaway, Jeff A Ranish, James T Kadonaga, Paul Russell, Dong Wang
JoVE (Journal of Visualized Experiments) 2014, e51721
Biochemical assays for analyzing activities of ATP-dependent chromatin remodeling enzymes
Lu Chen, Soon-Keat Ooi, Joan W Conaway, Ronald C Conaway
JoVE (Journal of Visualized Experiments) 2014, e51720
Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
Lu Chen, Soon-Keat Ooi, Ronald C Conaway, Joan W Conaway
Proceedings of the National Academy of Sciences 2013; 110 (51), 20497-20502
Multiple modes of regulation of the human Ino80 SNF2 ATPase by subunits of the INO80 chromatin-remodeling complex
Lu Chen, Ronald C Conaway, Joan W Conaway
Journal of Biological Chemistry 2011; 286 (13), 11283-11289
Subunit Organization of the Human INO80 Chromatin Remodeling Complex AN EVOLUTIONARILY CONSERVED CORE COMPLEX CATALYZES ATP-DEPENDENT NUCLEOSOME REMODELING
Lu Chen, Yong Cai, Jingji Jin, Laurence Florens, Selene K Swanson, Michael P Washburn, Joan Weliky Conaway, Ronald C Conaway
Highlighted in “Milestones in transcription and chromatin published in the Journal of Biological Chemistry”, 2019 Feb 1;294(5):1652-1660.
Journal of Biological Chemistry 2013; 288 (36), 26179-26187
Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6α
Dotan Sela, Juliana J Conkright, Lu Chen, Joshua Gilmore, Michael P Washburn, Laurence Florens, Ronald C Conaway, Joan Weliky Conaway
Journal of Biological Chemistry 2012; 287 (27), 23035-23045
Endoplasmic Reticulum Stress-responsive Transcription Factor ATF6α Directs Recruitment of the Mediator of RNA Polymerase II Transcription and Multiple Histone Acetyltransferase Complexes
Dotan Sela, Lu Chen, Skylar Martin-Brown, Michael P Washburn, Laurence Florens, Joan Weliky Conaway, Ronald C Conaway
Publication
@Wuhan University /
Institute of Biophysics, CAS
Arteriosclerosis, Thrombosis, and Vascular Biology. 2007; 27:2005–2013
Cholesterol-dependent and-independent CD40 internalization and signaling activation in cardiovascular endothelial cells
Jianjun Chen, Lu Chen, Gang Wang, Hong Tang
